Aseptic Techniques

6 June 2017

The hospital environment could also be a source of infection hence measures have been taken to try and reduce the risks by regular disinfection and sterilization, providing good ventilation to the rooms, designed infrastructures that cater for areas here strict aseptic procedures and strict protocols are followed such as washing and disinfecting hands after each patient contact to prevent cross contamination from one patient to another, infected patients are isolated, single use of equipment and proper sterilization of non disposable equipment.

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Microbial contamination could cause physiochemical changes of the medicines. This could alter the contents of the active ingredients or convert them into toxic products. ” In research laboratories where cell cultures are grown there Is a high risk of contamination by micro-organisms the sources here could be the cells used In ultures, the glassware or apparatus although they have been autoclaved; Improper maintenance or sterilization of autoclaves may cause serious contamination out breaks In Industries at large.

As Stacey (2011) mentions that attention to tralnlng of staff, laboratory layout, appropriate use of quarantines for new cultures or cell lines, cleaning and maintenance and quality control are Important In preventing contamination in cell culture laboratorles. lll In this experiment we investigate the sources of contamination in the laboratory and how aseptic techniques and correct handling of microorganisms can help infection ontrol. Method: Please refer to the module handbook.

Results Activity A: Correct handling of Micro-organisms (Aseptic Technique) Table 1: Growth on plates Types of cultures streaked on to Nutrient Agar Escherichia coli Serratia Amount of growth Sectors 0-1 Sectors 1-2 Sectors 3 Sector 3 Sectors 4 sector 4 Were single colonies obtained? Yes Number of colony types Key O No Growth Occasional colonies Light Growth Moderate Growth Heavvy Growth Table 1 . Shows the growth of cultures of the nutrient agar plate that was streaked with E. coli.

The plate produced growth as follows; sectors 0-1 heavy growth, sectors -2 there appeared to be moderate growth of colonies, sectors 3 appeared to have occasional colonies produced while sector 4 had no growth at all. Also from the table it can be seen there was culture growth on the nutrient agar plate that was streaked with Serratia. The results for this were as follows; sector 0-1 produced heavy growth, sector 0-2 appeared to have a heavy growth of colonies, sector 3 had a light growth and sector 4 appeared to have occasional colonies.

Table 2: Growth in broth cultures Brotn cultures Growth in broth cultures Sterile nutrient broth A Loop transfer of sterile broth A to sterile broth B Inoculation of sterile broth with Escherichia coli. By loop transfer. C O No growth ( broth as clear as uninoculated broth). + Slight visible growth. ++ Moderate growth- distinct turbidity but you can still see through the tube. +++ Heavvy growth- the tube is so turbid so you can not see through it.

Table 2 Shows the results for the loop transfer of sterile broth (A) to sterile broth (B) and the inoculation of sterile broth (A) with bacteria (Escherichia coli) on nutrient agar plates, broth culture C. Here it can be seen that the sterile nutrient broth A had no growth of cultures at all. Loop transfer of sterile broth A to sterile broth B, had a slight visible growth and broth culture C which was the inoculation of sterile broth A with E. coli by loop transfer had a heavy growth. ource 0T contamlnatlon Type of medium (Aga r) (MCP/30mtn) Surface of bench before sanitizing (CFU/100cm2) Surface of bench after sanitizing Human Body (Female) (1. 7 m2) Nutrient 31 Sabouraud Activity B : Investigation of sources of microbial contamination. Table 3: The number of colonies on each plate: From Table 3. It can be observed that the nutrient agar in all the different conditions produced growth of colonies. However, it can be noted that the Sabouraund agar did not have growth of colonies at all.

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