Biology Ia Design

9 September 2016

Therefore, as more betalain diffuses out of the membrane into the water, the red pigments will turn the water red according to the amount of pigments that passes through the membrane. The red pigments will also diffuse through the membrane because the water has higher water potential than the beetroot piece. As the temperature is higher, more bonds will break and more betalain will diffuse out of the membrane, causing a darker colour. Which is why as the temperature is low, it will have an almost see through mixture because the bonds have not brake which means it is not able to diffuse through the membrane of the beetroot.

A further investigation could be carried out to prove this point by using another plant with pigments like the skin of a mangosteen, which contains purple pigments called anthocyanin. (This will only work if the properties of the plant is similar to the properties of a beetroot, for example, Dry and hard outer membrane). The same method could be used but if the results shows that as temperature increases the percentage of light transmitted will be lower, it means that what I mentioned in the previous paragraph will be true.

Biology Ia Design Essay Example

Therefore, my hypothesis is correct, as the temperature of the beetroot increases, the percentage of blue light shone through the mixture will be lower because more red pigments will pass through the membrane, which makes the mixture of water and belatain a darker and less transparent colour. Evaluation: As you can see in the graph of my processed data, the error bars in the graph represent the standard deviation of my data. Standard deviation means the accuracy of your results, which also means how spread out (not around the same values) your results are.

If the number you have for standard deviation is high, it means that your results are very spread out, which means that your data is not very reliable as it is not accurate. For the temperatures of 30 C° and 70 C° in my graph, the standard deviation is below 2 which shows a small error bar in the graph. That means that these two results where temperatures of 30 C° and 70 C° are the most reliable out of all as the total results are not very far apart from each other. For temperatures 55 C° and 8 C° the standard deviation is 4. 5 and 3.

The discs are not in the water bath at the same time for the ones in 40? C, this will affect the results because as I started the time, I could only have hold 3 test tubes at once, which means that after I put the first 3 test tubes into the water bath, I needed another 20 seconds to bring the other 2 test tubes and put it into the water bath, which could be the reason for the point at 35? C because my results were unfair. 5. While using the tissue to clean the beetroots after being washed, some of the red pigments passes through the membrane and gets absorbed by the tissue, which will affect the results.

It will affect the results because the number of betalain in the beetroot will decrease as it gets absorbed into the tissue, therefore less betalain will pass into the water. Improvements for the experiment Problem| Improvements| 1. Size of beetroot| We could try to cut the size of the beetroot accurately with a scalpel and a ruler under it, but this time cut 10 pieces instead of 5 so that you can get 5 sizes of the same. Or you can attach two scalpels together but 1cm apart, so that every time you cut the beetroot it is 1cm long.

Surface area of beetroot| While we are extracting the beetroot with a cork borer, try to do it smoothly. After extracting it, make sure to check if the surface is smooth and even, if it is not we should re-extract the beetroot again. | 3. Timing of the washing| 5 test tubes with the same amount of water in it should be ready before the 5 minutes time is up. Therefore when the time is up, you could just stop the water and put the discs in the test tube immediately instead of doing it after stopping the water. | 4.

Delay of test tubes in water bath| By using a 500ml beaker, you can fit in all 5 test tubes and place it in the water bath at the same time so you can time the heating process with a same amount of heating time. | 5. Tissue absorbing betalain| Put the tissue on the table and the beetroot on top of the tissue, so it will absorb the water in beetroot. Do not rub or roll the beetroot in the tissue because betalain will be absorbed by the tissue. | Equipment that could be used for the next time: A large beaker for the test tubes

Two sharp scalpels and a precise ruler that measures up to 1mm so that we can create the tool that cuts a 1cm thick disc. Further improvements for data recording: More temperatures could be investigated, maybe we should start at 10 ? C and go up with intervals of 10, this will give us a smoother line which will show a clearer pattern of the graph, which also increases the reliability of our result. Also, since the results might be far from each other (spread out), we could include more readings in a set of data, so maybe 10 readings per temperature in order to achieve a better average and a lower standard deviation.

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