Pinesol and Lysol are both common household disinfectants that make very big commercial claims; both claim to kill 99. 9 percent of bacteria. Lysol contains Phenol which is a highly volatile organic compound. Phenol is a carbolic acid found in some household cleaning supplies. Phenol is very toxic and because of this it is designed to kill bacteria. The phenol within the Lysol causes the environment where the bacteria thrive to be uninhabitable. Pinesol has several other organic compounds in it as well but none as volatile as Phenol, yet some consumers say that Pinesol is just as useful as Lysol.
Pinesol contains allkyl and isophryll acohol which are both do not disrupt the environment of the bacteria as effectively. variables Measurement range Independent Lysol and pinesol Concentration 2-3 milliliters dependant Bacterial growth colonies 5-7 Colonies Controls Uses how Agar plate The same agar allows this specific type of bacteria to grow; and adds consistency to environment. Use same type of agar plates. moisture Allows researcher to know how bacteria reacts with condensations Use same incubator for storage temperature Adds consistency to environment and gives bacteria optimum environment for growth.
Biology ia Essay Example
Use same incubator for storage Type of bacteria Adds consistency for experiment and is a bacterium Use same type of bacteria (E. Coli) Hypothesis: I think the Phenol in the Lysol will cause the most significant reduction in bacterial growth, because the Phenol is a volatile organic compound capable of bacterial reduction in extensive amounts. Materials: • 1 bottle of Lysol • 1 bottle of Pinesol • 2 graduated cylinders • 2 blood agar plates • sterilized cottonswabs • 2 incubator rods • 2 petri dishes Procedure: 1) Wash your hands 2) Label the top of the container with your initials and name of household cleaner that is going to be used on the Streptococcus Baccillius. 3) Begin by sterilizing the petri dishes. Accomplish by placing them in a bath of boiling water and allowing to dry upside down on a sanitized drying rack or lab work bench. If the Petri dishes are still enclosed in the original packaging, leave them until ready to use. For 500 milliliters (ml) of agar mixture, you can fill 25 average-sized petri dishes. 4) Prepare agar powder by placing it in the microwave with the appropriate amount of water. The label should provide specific directions although 6. 9 grams to 500 ml of water (or just a little more than two cups) is standard. For agar tablets, 10 tablets dissolved in two cups of water is standard. 5) Treat bottled agar formula carefully by loosening the bottle cap (but not removing) before placing in the microwave to warm and soften into a liquid state. Sterilize the bottle after microwaving by placing the neck of the bottle over an open flame a few times to ensure any airborne germs are eradicated before proceeding to plate making. 6) Place the petri dishes the right way up and crack but do not remove the lids.
This is to prepare them for individual handling when the other hand is holding the agar mixture in a suitable pouring vessel such as a glass pitcher. 7) Gently lift the lid of the petri dish with one hand and hover the lid immediately over the bottom dish to remove any chance of airborne germs entering. 8) Pour an amount of agar liquid into each dish as you hover the lid as mentioned in the previous step. About 1/8 inch is a standard thickness for the liquid measure. 9) Allow the agar to set into the dishes by leaving them on a flat, clean surface to dry in the natural room temperature.
Secure lids once set and store upside down. 10) After agar plates are prepared and ready to use . Take the sterile cottonswab out of the wrapping. 11) Place sterile cottonswab in the Streptococcus bacterial culture, and rotate the cottonswab in a clockwise motion for 25 seconds . 12) Extract the cottonswab carefully from the bacterial culture slowly and carefully 13) Wipe the extracted cottonswab making sure you do not press to hard as to make on impression in the agar. Wipe the cottonswab from one end of the plate to the other making a zigzag all the way down the Plate.
14) Throw away the cottonswab in hazardous waste bin 15) Seal the plate and place the place agar plate having the agar facing downward in incubator at 98*F . 16) Wait 24 hours 17) After 24 hours, get a metal rod with a loop at the end and turn on the Bunsen burner . 18) Put the end of the metal rod with the loop on the Bunsen burner and keep it there for 10 seconds. 19) Let the metal rod cool for 30 seconds 20) Use the metal rod to carefully and light scrape 5 colonies and place them onto a Petri dish 21) Pour 2mL Of Lysol onto the colonies 22) Wait 25 minutes
23) Repeat all steps with Pinesol 24) Create a comparison chart between the 2 household disinfectants and the amount of colonies in the Petri dish before and after the colonies came in contact with the disinfectant Growth before application of Chemicals trials control Lysol Pinesol 1 30 colonies 31 colonies 35 colonies 2 40 colonies 37 colonies 34 colonies 3 33 colonies 35 colonies 37 colonies 4 41 colonies 38 colonies 39 colonies 5 19 colonies 23 colonies 21 colonies Growth after application of Chemicals Trials Control Lysol Pinesol 1 35 colonies 0 colonies 36 colonies 2 41 colonies 1 colony 37 colonies 3 38 colonies 0 colonies 40 colonies 4 42 colonies 0 colonies 40 colonies 5 20 colonies 1 colony 22 colonies Annotations for experiment • I had to wait 48 hours instead of 24 hours for bacterial growth because in 24 hours the growth was too little to be examined. • During the 5th trial the plates grew mold so I had to redo the experiment. • In the first control I analyzed it grew throughout all 5 trials and after the 5th trial the colonies grew so exponentially that the agar decreased in size. •
The Lysol had the greatest bacterial deduction and in Pinesol it actually caused an increase in bacterial growth after application. • In the 5th trial I used a different type of agar which was most likely the cause of the overall decrease in growth • I had to change the bacteria used in the experiment because it was taking too long for the Streptococcus Bacillus to be shipped. • I had to use standard agar instead of blood agar also because of cost and shipping complications. Data Manipulations Standard Deviation of Control Before Chemical Application: ((30 – 32. 6)2 + (40 – 32. 6)2 + (33 – 32. 6)2 + (41 – 32. 6)2 + (19 – 32. 6)2)/5 = 8. 9 Standard Deviation of Control After Chemical Application: ((35 – 35. 2)2 + (41 – 35. 2)2 + (38 – 35. 2)2 + (42 – 35. 2)2 + (20 – 35. 2)2)/5 = 8. 9 Standard Deviation of Lysol Group Before Chemical Application: ((31 – 32. 8)2 + (35 – 32. 8)2 + (37 – 32. 8)2 + (38 – 32. 8)2 + (23 – 32. 8)2)/5 = 6. 9 Standard Deviation of Lysol Group After Chemical Application: ((0 – 0. 4)2 + (1 – 0. 4)2 + (0 – 0. 4)2 + (0 – 0. 4)2 + (1 – 0. 4)2)/5 = 0. 55 Standard Deviation of Pinesol Group Before Chemical Application: ((35 – 33.
2)2 + (34 – 33. 2)2 + (37 – 33. 2)2 + (39 – 33. 2)2 + (21 – 33. 2)2)/5 = 7. 1 Standard Deviation of Pinesol Group After Chemical Application: (36 – 35)2 + (37 – 35)2 + (40 – 35)2 + (40 – 35)2 + (22 – 35)2/5 = 7. 5 ? Conclusion: ?Through this experiment, I was able to prove that my predicted hypothesis was accurate. The Phenol within the Lysol virtually killed all the bacteria grown on the petri dish. On the other hand, the pinesol only hindered by did not prohibit the bacterial growth. This shows that not all household cleaners accurately prohibit bacterial growth as advertised.
This experiment was relative straightforward in relation to procedure, although there was some minor interference. I had to wait 48 hours instead of 24 hours for bacterial growth because in 24 hours the growth was too little to be examined. It became rather humid one day during the trials, so during the 5th trial the plates grew mold so I had to redo that trial. Also In the first control I analyzed it grew throughout all 5 trials and after the 5th trial the colonies grew so exponentially that the agar decreased in size, which affected the growth of the bacteria because if there was more agar then the growth would have been more.
These factors affected the experiment and are noteworthy but did not compromise the whole experiment. ?Though this experiment was relatively accurate, if I could do this experiment again I would try to measure the effects of Lysol on bacterial growth. I would blood agar and standard and see how the growth would be affected by using both of these types and I would use staphylococcus instead of E. Coli. ? The bacterial growth before the Lysol was applied to the petri dish ? The bacterial growth after application of Lysol