The purpose of this lab was to perform and understand the procedures of conducting an ELISA test to determine whether a particular antibody is present in a patient’s blood sample through a virtual simulation. Hypothesis: If I successfully complete this lab, I will then understand how to perform an ELISA test, the purpose an ELISA test, and also how to interpret the results of this test. Materials and Procedures: Materials: Howard Huges medical Institute: Virtual Immunology Lab Human Anatomy & Physiology Marieb 9th edition textbook
Web access Procedures: Use the hyperlink Virtual Immunology Lab to visit the website where the virtual lab is located Click on the image to the left of the summary to launch the lab Read the diagnosis and background for the lab before you begin Follow the instructions provided throughout the lab BE SURE to pay close attention and follow each step precisely When you have completed the entire lab, copy the data results into the data & analysis section of your report Data & Analysis: Conclusion: The hypothesis stated was correct.
After completing this lab I now understand how to perform an ELISA test, the purpose, and also how to interpret the results. An ELISA or Enzyme-linked Immunosorbent Assay test uses components of the immune system and chemicals to detect immune responses in the body (for example, to infectious microbes) and involves an enzyme and antibody or antigen (immunologic molecules). The purpose of an ELISA test is to detect substances that have antigenic properties, primarily proteins, which include hormones, bacterial antigens, and antibodies (MedicineNet, 2005).
If done correctly, an ELISA test can confirm whether or not a patient has a particular disease or if there is a possibility that they could develop it. It is very important that a test such as this is performed correctly and repeated a few times just to be sure that the results aren’t a false-positive or false-negative. Notice in the lab results above that the ELISA plate was not washed like it should have been, thus yielding a false-positive indicating that all three patient’s may possibly have SLE.
If all steps of the experiment would have been done correctly, then the results would have been as follows: patient A is likely to have SLE, patient C is likely to NOT have SLE, and patient B may have SLE, but further testing needs to be done. ELISA testing is very helpful, but it is not the final confirmation due to a variety of limitations that it has. Further testing and other factors would need to be taken into account before confirming whether or not a patient has a particular disease.