What were we trying to accomplish by amplifying this region of DNA from the nuclear genome? Why do we care if our class is in Hardy Weinberg Equilibrium? More generally, why do we care if any population is in Hardy Weinberg Equilibrium? 2. Methods: You do not need to rewrite everything you did step by step. But you should give a general overview of the techniques you used. For example, rather than stating step by step the DNA extraction process, you could say something like “we extracted our DNA from cheek cells using proteinase K digestion and a silica spin column”.
If you deviated from the protocols given to you in class you must state what you did differently. For example, if you accidentally pipetted 2 ? l of pro K instead of 20 ? l and then added additional pro K to compensate for this error, state it in the Methods section. The Methods section should include everything from the DNA extraction to the ? 2 analysis. 3. Results: This is the section where I expect you to be the most detailed. What did your gels show? Did every PCR reaction work? What did your negative controls show? Did you use your own data or data provided by Prof. Dolcemascolo?
What were your frequencies for the 100 bp allele and the 400 bp allele? How did you arrive at these estimates? What were your expected/observed absolute and relative frequencies of all three genotypes? What were your X2 and p-values? This section should be where you report what you actually observed and/or calculated: your results. You should not report any interpretation of these results in this section. 4. Discussion: This is the section where you should discuss the interpretation of your results. Was the class is Hardy Weinberg Equilibrium? If so, what does that mean? If not, why do you think the class is not in equilibrium?
Do you think the class is representative of all humans? If anything did not work (like a failed PCR reaction), why do you think it didn’t work? Note: general, ambiguous answers like “human error” or “technical error” are not acceptable. How do you think your results may change if you used a different locus (one that codes for an amino acid sequence for example)? 5. General notes: Please make sure to properly cite any outside resource you use. You should have at least 2 PEER-REVIEWED outside resources (my protocols and/or class lectures do NOT count). If you do not know what a peer-reviewed source is, please talk to Prof.
Dolcemascolo for help. Note: Do NOT use web sites as resources as most of them are not acceptable. This is especially true for web sites like Wikipedia which are not monitored that well for accuracy (although they are a great place to find potential resources). There is not a specific length requirement. Just take as long as you need to explain your ideas. If you can write concisely and address all of the points listed above, then that’s great! Reports must be typed or computer-printed, and please use 12 point font and single line spacing. If you use someone else’s idea, you MUST cite them properly. Yes, grammar and spelling count!